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. 2009 Jul 14;284(37):24981–24987. doi: 10.1074/jbc.M109.011973

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Quantitative real-time RT-PCR analysis of PRC2, pluripotent, and developmental genes expression. A and B, the differentiation-related genes (Cdx2, GATA6, HAND1, HoxB1, and HoxB4), were up-regulated significantly in cloned embryos compared with normal embryos at blastocyst and late blastocyst stages. NT-B, cumulus cell SCNT-produced day 3.5 blastocysts; F-B, fertilization-produced day 3.5 blastocysts; L-NT-B, cumulus cell SCNT-produced day 4.5 blastocysts; and L-F-B, fertilization-produced day 4.5 blastocysts. C, expression comparison of pluripotent genes of embryos at the blastocyst stage. SCNT-produced embryos showed similar expression level of Nanog and Sox2 compared with fertilization-produced embryos; however, Oct4 expression was significantly reduced in clones. D and E, the PRC2 components (Eed, Ezh2, and Suz12) responsible for H3K27me3 were down-regulated in cloned embryos, especially at the morula stage. NT-M, SCNT-produced day 2.5 morulae; F-M, fertilization-produced day 2.5 morulae. *, p < 0.05; **, p < 0.01; and ***, p < 0.001.