TABLE 3.

Kinetic parameters of recombinant AtD-LDH and AtD-2HGDH

Kinetic data were best fit by nonlinear regression analysis. Parameters represent the mean ± S.E. of two independent enzyme preparations.

	With cytochrome c
	Km	kcat	kcat/Km
	μm	min−1	min−1mm−1
AtD-LDH
d-2HB	61 ± 2	88 ± 1.2	1450
d-lactate	164 ± 11	65 ± 1.8	395
l-lactate	4486 ± 374	7.0 ± 0.3	2.0
d-glycerate	8871 ± 1379	6.0 ± 0.8	1.0
Glycolate	432 ± 31	0.1 ± 0.0	0.2

	With DCIP
d-lactate	317 ± 31	73 ± 1.7	231
l-lactate	7134 ± 456	5 ± 0.1	0.7
Glycolate	596 ± 44	0.4 ± 0.0	0.7
AtD-2HGDH
d-2HG	584 ± 45	48 ± 0.9	82