TABLE 1.
Complementation tests of DnaA F46A in vivo
KH5402-1 (wild type) cells were transformed with mini-R derivative plasmid bearing wild-type rnhA and wild-type dnaA, dnaA F46A allele, or none and incubated at 30 °C for 20 h on LB agar plates containing thymine (50 μg/ml) and ampicillin (100 μg/ml). KA451 [dnaA ::Tn10 rnhA ::cat] and KK001 [dnaA ::Tn10 rnhA ::cat ΔdiaA] cells were similarly transformed and incubated for 36 h as above.
| Strain | Relevant genotype |
Transformation efficiency |
||||
|---|---|---|---|---|---|---|
| rnhA | dnaA | diaA | pRRNH (none) | pOZ18 (wild-type dnaA) | pRF46A(dnaA F46A) | |
| ×106 transformants/μg DNA | ||||||
| KH5402-1 | + | + | + | 1.4 | 1.1 | 1.4 |
| KA451 | − | − | + | <2.8 × 10−4 | 1.5 | 0.68 |
| KK001 | − | − | Δ | <2.8 × 10−4 | 1.9 | 0.41 |