TABLE 1.
13C NMR chemical shifts in CsgA fibrils determined from the two-dimensional spectrum in Fig. 8C
Random coil values (36) were converted to the tetramethylsilane chemical shift reference by subtraction of 1.7 ppm.
| Residue type | Cα chemical shift | Cα random coil value (36) | Cβ chemical shift | Cβ random coil value (36) | Predicted secondary structure |
|---|---|---|---|---|---|
| ppm | ppm | ||||
| Alanine (major) | 48.9 | 50.8 | 20.8 | 17.4 | β |
| Alanine (minor) | 53.2 | 50.8 | 16.9 | 17.4 | Non-β |
| Valine (major) | 58.4 | 60.5 | 33.3 | 31.2 | β |
| Valine (minor) | 59.7 | 60.5 | 30.7 | 31.2 | Non-β |
| Asparagine/aspartate | 52.1 | 51.4/52.5 | 37.7 | 37.2/39.4 | Non-β |
| Glutamine/glutamate | 52.1 | 54.0 | 31.2 | 27.7 | β |
| Serine | 55.4 | 56.6 | 64.2l | 62.1 | β |
| Threoninea | 59.9 | 60.1 | 68.8 | 68.1 | β |
a The threonine CO chemical shift is 171.2 ppm, whereas the random coil value is 173.0 ppm.