FIGURE 1.
Pif1 stimulates strand displacement synthesis by pol δ. Strand displacement synthesis by pol δ (23 fmol) was assayed on the standard substrate (U2:T2:D2) in the presence of various combinations of PCNA (25 fmol), RFC (25 fmol), and varying amounts of Pif1 (25, 50, or 100 fmol) as indicated in the figure and as described under “Experimental Procedures.” The substrate is depicted above the figure, with the location of the radiolabel indicated by the asterisk. Plus (+) denotes presence and minus (−) denotes absence of the given enzyme.