TABLE 1.
Oligonucleotide sequences
| Primer | Length | Sequence |
|---|---|---|
| nt | ||
| Upstream (5′–3′) | ||
| U1 | 20 | GTCCACCCGACGCCACCTCC |
| U2 | 44 | GTCCACCCGACGCCACCTCCTGCCTTCAATGTGCTGGGATCCTA |
| Downstream (5′–3′) | ||
| D1 | 29 | ACCGTGCCAGCCTAAATTTCAATCCACCC |
| D2 | 60 | AGACGAATTCCGGATACGACGGCCAGTGCCGACCGTGCCAGCCTAAATTTCAATCCACCC |
| D3 | 90 | TTCTACTTCCAATTGATACGCGCTATAACCAGACGAATTCCGGATACGACGGCCAGTGCCGACCGTGCCAGCCTAAATTTCAATCCACCC |
| D4 | 95 | TTCTACTTCCAATTGATACGCGCTATAACCAGACGAATTCCGGATACGACGGCCAGTGCCGACCGTGCCAGCCTAAATTTCAATCCACCCTGACT |
| Template (3′–5′) | ||
| T1 | 42 | CAGGTGGGCTGCGGTGGAGGGTCGGATTTAAAGTTAGGTGGG |
| T2a | 110 | CAGGTGGGCTGCGGTGGAGGACGGAAGTTACACGACCCTAGGATGTTGGTTCTGCTTAAGGCCTATGCTGCCGGTCACGGCTGGCACGGTCGGATTTAAAGTTAGGTGGG |
a Template T2 is tinylated at both the 5′ and 3′ ends.