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Characterization of HlyA and Pro-HlyA cysteine mutants. A, specific hemolytic activity of the different cysteine mutant proteins (n = 3) compared with the wild type toxin. HU, hemolytic units. B, emission spectra of Trp of wild type (WT) HlyA (solid black line), HlyA K344C (light gray dashed line), Pro-HlyA K344C (dark gray dashed line), and Pro-HlyA (black dotted line). The amount of toxin measured was of 10 g/ml, λexc was 295 nm.
