FIGURE 7.

The T384A mutation results in a dramatic reduction of the apparent affinity of SNAT2 for Na⁺. The apparent affinities of SNAT2_WT (A) and SNAT2_T384A (B) for extracellular Na⁺ were measured by recording 20 mm l-alanine-induced transport current as a function of extracellular Na⁺ at a membrane potential of 0 mV (140 mm Mes⁻, extracellular solution, 140 mm KMes intracellular solution).