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Mutational analysis of the FBP21 tandem WW domains. Combined chemical shift changes upon ligand binding are calculated as in Equation 1; the mutants V8A, E9A, W29A, and W70A are colored as shown, secondary structure elements are represented by gray boxes. A, peptide P1 (RGPPPPGMRPPRP) added to the FBP21 tandem WW domains and indicated mutants. B, peptide P2 (GPPPRGPPPR) added to the FBP21 tandem WW domains and indicated mutants. WT, wild type.
