TABLE 2.
Interactions between FBP21 tandem WW domains and proline-rich peptides
| Motif | Peptide | Protein | Sequence | Bindinga |
|
|---|---|---|---|---|---|
| WW1 | WW2 | ||||
| Group III | P1 (13AA) | SmB′-(221–233) | RGPPPPGMRPPRP | +b | + |
| PPR/PGMc | P2 (10AA) | SIPP1-(481–490) | GPPPRGPPPR | + | + |
| P3 (10AA) | SIPP1-(501–510) | PPRPGMMRPP | + | + | |
| Group II | P4 (9AA) | Leukocyte formin-(541–549) | APPPPPPLP | + | + |
| PPLP/PPPPc | P5 (10AA) | DIAPH3-(589–598) | PPPPPPPPPP | + | + |
| P6 (10AA) | SIPP1-(491–500) | LPPPAPPGIP | + | + | |
| P7 (20AA) | SIPP1-(481–500)(p2+p6) | GPPPRGPPPRLPPPAPPGIP | + | + | |
a The interactions between FBP21 tandem WW domains and proline-rich peptides were studied using NMR chemical shift perturbation experiments.
b The proline-rich peptides bind to the WW domains.
c The proline-rich motifs in peptides are shown in boldface.