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. 2009 Sep 2;29(35):10788–10801. doi: 10.1523/JNEUROSCI.2132-09.2009

Figure 1.

Figure 1.

Biochemical and immunohistochemical characterization of APP expression and localization. A, Western blot analysis of APP protein levels from P0 spinal cord or muscle samples of APLP2-null control (Ctrl), neuronal or muscle conditional APP knock-out on APLP2-null background (N-dCKO and M-dCKO, respectively) using the APPc antibody. Tubulin was used as a loading control. B, Immunofluorescence staining of P0 stenomastoid muscle sections of control (Ctrl), APP/APLP2 double knock-out (dKO), N-dCKO, or M-dCKO animals using the anti-APP antibody Y188. α-BTX staining was used to mark the postsynaptic AchRs. Merge, Overlay of APP and AchR images. Scale bar, 20 μm. C, Quantification of APP signal intensity (mean ± SEM) (2 animals/genotype; 6 sections/animal) in AchR-positive endplates. **p < 0.01, ***p < 0.001 compared with control (one-way ANOVA).