Figure 5. Cytokine production by Th1 or Th2 differentiated T-CD4 T cells deficient in signaling molecules.
The same chimeric mice described in Figures 1-3 were used to prepare splenic CD4 T cells. Cells were cultured under the Th1 or Th2 skewing condition, restimulated for 5 hours, and analyzed for IFN-γ and IL-4 production as described in Experimental Procedures. Cytokine production by T-CD4 T cells deficient in SAP (A), PKCθ (B), and T-bet (C) are shown.