A. The patient's HIV subtype and the nature of the amino acid in position 316 in PBMC (PB) and cervix (Cx) is listed; A= Alanine, M= Methionine, S-= Serine, T= Threonine, and V= Valine (n=38, as the dually-infected woman is shown as both subtype-A and -C).
B. Phylogenetic tree demonstrating genital selection of 316T (red circle) and blood selection of 316A (blue circle) in a subtype-B infected woman. Bootstrap estimates above 60 are shown. Statistically significant values are generally considered to be ≥70, although no established significance threshold exist.
C. Percentage of different V1-V2 loop lengths in gp160 sequences from vagina, cervix and blood from a subtype-C infected woman with V3-316T.