Figure 5.
Halo-formation assay for assessing the antibiotic activity of 2, 3 and 14. A tryptic soy agar plate plated with B. subtilis was spotted with (a) 2, (b) 3 and (c) 14. Each well contained the corresponding compound at an incremental amount of 2: 1.0 ng; 3: 2.0 ng; 4: 4.0 ng; 5: 8.0 ng and 6: 16 ng. Well 1 is a negative control.