Figure 2.

ILK expression occurs in granule precursor cells and Bergmann glial cells of the EGL. Sagittal sections of the cerebellum of P10 mice were stained with antibodies to ILK (green) and GFAP (red) (a–c). Although ILK immunoreactivity (b) appears to colocalize with some GFAP-positive cells (c), a large number of ILK-positive cells are GFAP negative, appearing as a honeycomb staining pattern within the cerebellar folium (b). c, Inset, A high-magnification view of glia end feet is shown, illustrating costaining for both ILK and GFAP. ILK immunoreactivity colocalizes with the proliferation zone of the EGL (d–f). Sagittal sections of P10 wild-type mice (that were previously injected with BrdU) were costained with antibodies to ILK (red) and BrdU (green). ILK-immunoreactive cells (d) occurring near the basement membrane were also BrdU positive (e). f, Inset, A high-magnification view of cells within the proliferating zone (BrdU-positive cells) that costain for ILK. Scale bars, 50 μm.