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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Gastroenterology. 2009 Aug 3;137(4):1380–1390.e3. doi: 10.1053/j.gastro.2009.07.004

Figure 2. SPF TLR4-/- x IL-10-/- mice exhibit an exacerbated form of the Th17 driven colitis previously reported in IL-10-/- mice.

Figure 2

(A) Immunohistochemical staining for CD4+ T cells in colonic tissue of TLR4-/-, IL-10-/-, SPF TLR4-/-x IL-10-/- and Hpp free TLR4-/- x IL-10-/- mice, 20X. (B). Cytokine secretion into 48 hr. anti-CD3/anti-CD28 stimulated supernatants was examined by either ELISA or Luminex assay. Bar graphs represent the mean +/- SEM of duplicate or triplicate samples from cultures of individual mice; TLR4-/- (white, n = 4), IL-10-/- (light gray, n = 5), SPF TLR4-/- x IL-10-/- (DKO, dark gray, n = 7), and Hpp free TLR4-/- x IL-10-/- (DKO, black, n = 5).