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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Gastroenterology. 2009 Aug 3;137(4):1380–1390.e3. doi: 10.1053/j.gastro.2009.07.004

Figure 4. Activated CD4+Foxp3+Tregs from the spleen and MLN of TLR4-/-, IL-10-/- and TLR4-/- x IL-10-/- mice secrete inflammatory cytokines but retain functional suppressive activity.

Figure 4

(A) In vitro suppression assay. CD4+Foxp3+ T cells sorted from the spleen and MLN of the mice analyzed in Figure 3 were co-cultured in triplicate with irradiated splenic APC, anti-CD3 and CD4+Foxp3- T cells sorted from the same tissue. (B) Cytokine/chemokine production by each of the sorted CD4+Foxp3+ Treg populations was assayed 72 hrs. after stimulation with anti-CD3/anti-CD28 from 2-3 replicate cultures using multiplex analysis. * = p < .05