. Author manuscript; available in PMC: 2010 Sep 1.

Published in final edited form as: Mol Pharm. 2009 Sep–Oct;6(5):1311–1320. doi: 10.1021/mp900011h

Table 1.

Physico-chemical properties of TAT-conjugated nanoparticles (NPs) and their effect on the change in surface pressure (SP) of the endothelial model cell membrane (EMM).

Formulation	TAT peptide added for conjugation^* (μg)	TAT molecules conjugated/NP	Mean NP Size in nm (P.I.)	ζ-Potential (mV)	ΔSP^** (mN/m)
RNPs (without epoxy activation)	-	-	234 (0.04)	−19.3 ± 0.3	0.9
TAT₂₀-RNPs	20	750	321 (0.04)	2.0 ± 0.2	3.9
TAT₂₀₀-RNPs	200	2042	338 (0.23)	3.5 ± 0.2	5.8
TAT₅₀₀-RNPs	500	3980	355 (0.20)	20.3 ± 0.7	9.0
sc-TAT₂₀₀-RNPs	200	2531	310 (0.18)	5.0 ± 0.5	0.5
Ritonavir alone	-	-	-	-	0.0
TAT peptide alone	-	-	-	-	0.0
sc-TAT peptide alone	-	-	-	-	0.0

Initial amount of TAT peptide added to 60 mg of ritonavir-loaded nanoparticles during conjugation. Numbers in subscripts for different formulations of conjugated NPs indicate the initial amount of TAT peptide added for conjugation. RNPs: Ritonavir-loaded NPs; TAT-RNPs: TAT peptide-conjugated RNPs; sc-TAT-RNPs, scrambled TAT peptide-conjugated RNPs; P.I., Polydispersity index.

^**

Change in SP of the EMM with respect to injection of water.