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. Author manuscript; available in PMC: 2010 Oct 6.
Published in final edited form as: FEBS Lett. 2009 Sep 6;583(19):3221–3224. doi: 10.1016/j.febslet.2009.09.009

Fig. 2.

Fig. 2

Kl is shedded by ADAM10, 17 and BACE1. A) MEFs cells derived from KO mice as indicated were transiently transfected with Kl-flag and shedding was assayed after 24h. After Western blotting, lysates and media were probed with Kl antibody. Representative gels are shown. B) Quantitation of shedding. The ratio of shedded Kl in the medium versus Kl in cell lysates was determined in all MEF lines. In ctrl MEF cells this ratio was set to 100% and the ratios from the other cell lines related to that. Error bars depict standard deviation from 4-7 independent experiments. ***, p<0.001; **, p<0.01.