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(A) Immunostaining of the CHO, HepG2, and BNL cell lysates with aC-WND or preimmune serum (HepG2, PreIm). (B) The intensity of the 140-kDa product in cells with different level of WND mRNA. Thirty micrograms of protein was loaded to each lane, except the lymphoblasts sample, in which 60 μg of protein was used. aC-WND dilution is 1:5,000. The lysate of HepG2 cells grown in the presence of 20 μm CuCl2 is labeled HepG2+Cu.
