Figure 2.

(A Upper) The full-length WND protein is expressed in COS cells as a 160-kDa protein. COS cells were transfected with the WND cDNA. Twenty-five micrograms of mock-transfected (−) and WND cDNA-transfected (+) cell lysates were analyzed and compared with HepG2 lysate. (B Upper) The longer exposure reveals a 160-kDa band in addition to the 140 kDa product in HepG2 sample. (Lower) The antibody aN-WND detects the recombinant N-terminal domain of the WND protein (A) and specifically recognizes the 140-kDa protein (B) and 16-kDa protein (C) in HepG2 cell lysates. The recombinant domain was obtained as described (13).