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(A) Identification of the 140-kDa WND product in human heart mitochondria (lane 1), skeletal muscle mitochondria (lane 2), and HepG2 (lane 3). Thirty micrograms of protein is in each lane. Identification of the 160-kDa and 140-kDa products in HepG2 lysates with JGD and aC-WND (5-min exposure) (B) or with JGD, a-ABD and aC-WND (30 sec exposure) (C). Twenty-five micrograms of protein is in each lane.
