Figure 6.
Effect of zinc deprivation on cellular lipid accumulation in HepG2 cell culture. HepG2 cells were treated with TPEN at 2 µM with or zinc supplementation at 25 or 50 µM for 4 days. A. Oil Red O staining of neutral lipid. B. Triglyceride concentrations. C. Free fatty acids (FFAs) concentrations. Results are means ± SD (n=4). Significant differences (p<0.05) between a and b are determined by ANOVA. Ctrl: control. T: TPEN.