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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2009 May 5;1788(10):2059–2068. doi: 10.1016/j.bbamem.2009.04.020

Figure 1. Assembly of ATP synthase oligomers, the respiratory supercomplex, and AAC2 complexes in the presence and absence of CL.

Figure 1

100 μg of 1.5% (wt/vol) digitonin extracts from mitochondria derived from wild type (+CL) or Δcrd1 (-CL) yeast were resolved by 2D BN/SDS–PAGE and immunoblots performed for complex V (F1α/β), complex IV (Cox2p), and AAC2. * highlights crossreaction with porin of the AAC antiserum. The migrations of the Vdimer, Vmonomer, III2IV2, III2IV, and IV supercomplexes are indicated schematically above the appropriate set of panels. The composition of the different AAC2 complexes is indicated above the AAC immunoblots. AAC1, ADP/ATP carrier isoform 1; Pic, phosphate carrier; Dic, dicarboxylate carrier; Ggc, GTP/GDP carrier. The interaction of AAC2 with the indicated mitochondrial carriers occurred in complexes ranging in size from ∼400-160 kDa. The exact size of each complex has not been determined; thus, the order of the depicted AAC2-carrier protein interactions is for illustrative purposes only. Details provided in text.