Figure 3.

A. NF-κB was activated in the liver nuclear extract of mice fed CDAA diet. Identical amount (3 μg) of the extract was incubated with ³²P-labed NF-κB oligo under optimal binding conditions. The protein DNA complex was resolved in a 5% polyacrylamide gel, dried and subjected to phosphorimager analysis. For competition and supershift assays, the extracts were preincublated with 100 fold molar excess of unlabeled oligos and antibodies, respectively for 30 minutes before adding labeled probe. B. Quantitative analysis of the data in A.