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. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: Hepatology. 2009 Oct;50(4):1152–1161. doi: 10.1002/hep.23100

Downregulation of C/EBPβ and PTEN, respective targets of miR-155 and miR-21, in the livers of mice fed CDAA diet. A. Schematic representation of conserved miR-155 site in C/EBPβ 3'UTR. B. Western blot analysis of C/EBPβ in HCC cells. Hep3B and HepG2 cells were transfected with pre-miR-155 (50nM) followed by Western blot analysis after 48 hours. C. Real-time RT-PCR analysis of C/EBPβ in the liver of mice fed diet for 32 and 65 weeks. D and E. Western blot analysis of C/EBPβ, PTEN and GAPDH in the liver extracts. Equal amount of proteins were subjected to immunoblot analysis first with specific primary and secondary antibodies and the signal was developed with ECL reagent. The signal was quantified using Kodak Imaging software and the data was normalized to GAPDH.

Downregulation of C/EBPβ and PTEN, respective targets of miR-155 and miR-21, in the livers of mice fed CDAA diet. A. Schematic representation of conserved miR-155 site in C/EBPβ 3'UTR. B. Western blot analysis of C/EBPβ in HCC cells. Hep3B and HepG2 cells were transfected with pre-miR-155 (50nM) followed by Western blot analysis after 48 hours. C. Real-time RT-PCR analysis of C/EBPβ in the liver of mice fed diet for 32 and 65 weeks. D and E. Western blot analysis of C/EBPβ, PTEN and GAPDH in the liver extracts. Equal amount of proteins were subjected to immunoblot analysis first with specific primary and secondary antibodies and the signal was developed with ECL reagent. The signal was quantified using Kodak Imaging software and the data was normalized to GAPDH.