Figure 5. Inhibition of Hh-signaling attenuates EMT-associated fibrogenesis in vivo.
WT mice were fed either normal chow (n= 4) or MCDE diet (n = 8) for 1 week with or without cyclopamine (n= 4/group; 0.6mg per mouse per day; i.p.). At the end of treatment, total liver RNA was harvested for QRT-PCR (a) gli2, (b) tgf-β, (c) bmp7, (d) vimentin, (e) mpk. Results are expressed as fold change relative to the respective chow-fed control group and graphed as mean ± SEM. * P < 0.05 vs control or MCDE group; **P < 0.005 vs control