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. Author manuscript; available in PMC: 2010 Sep 30.
Published in final edited form as: Adv Drug Deliv Rev. 2009 Aug 11;61(11):977–985. doi: 10.1016/j.addr.2009.07.014

Fig. 1.

Fig. 1

Principle of the ICC-ELISA. Immunotoxins, comprising the 38 kDa portion (domain II and III) of Pseudomonas exotoxin A fused to an Fv specific for target antigens on cancer cells, are incubated with candidate mAbs. Because the reaction takes place in solution, all epitopes on native PE38 are theoretically available. Complexes are captured to the plate with immobilized target ligands such as CD22-Fc or similar molecules. The primary antibody is then detected with an HRP-labeled secondary antibody. Reproduced with permission from The Journal of Immunology [published 2006, Vol. 177, 8822–8834] Permission pending.