Fig. 1.

PIN1 gene structure, reporter gene constructs for the PIN1 promoter and luciferase expression of the constructed promoter in different cell lines. (A) Genomic structure, locations and genotypes of three selected PIN1 SNPs. (B) Schematic drawing of the reporter gene constructs containing a 1016 bp PIN1 promoter region; the only difference between the two constructs was a G or C at the −842 polymorphic site. (C) Luciferase activity of the two PIN1 promoter constructs in three head and neck cancer cell lines: MDA1386ln, MDA886 and JHU011. Fold increase was measured as the activities of the reporter gene constructs relative to that of the empty pGL3 basic vector using the data (mean ± SD) from three independent transfection experiments: P < 0.001 for all comparisons of each cell line between the activities of the reporter gene constructs.