Fig. 4.

Production of a recombinant form of AftD in M. smegmatis mc²155 using the inducible pJAM2 system. (A) Growth characteristics of mc²155/pJAM2 (diamonds) and two independent mc²155/pJAMRv0236c clones (squares and triangles) in the MM63 medium before and after induction with acetamide (indicated by an arrow). (B) Production of a recombinant C-ter His₆-tagged form of AftD from M. tb 8-h post-induction with acetamide was detected by Western blot using a monoclonal anti-His tag antibody in the mc²155/pJAMRv0236c strain (lane 2) but not in the control strain, mc²155/pJAM2 (lane 1).