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Effect of overexpressing aftD on the lipoglycan content of M. smegmatis. Autoradiogram of the lipoglycans extracted from identical amounts of mc2155/pJAM2 and mc2155/pJAMRv0236c cells labeled with [U-14C]glucose and separated on a Tricine SDS–PAGE gel. The positions of products X and Y are indicated. Samples were either untreated or submitted to mild-acid (acid) or mild-alkali (alkali) treatments. MWM, molecular weight marker.
