Fig. 8.
RT-PCR analysis of the expression of aftD and adjacent genes in M. smegmatis mc2155 and the aftD single crossover strain SCO1. (A) Schematic representation of the aftD genomic region in the single crossover strain SCO1. The thin line symbolizes the body of the integrated plasmid, pPR27MSMEG_0359KX, and is not represented to scale. The lengths of the intergenic regions are indicated. (B) RT-PCR analysis of the sigA, MSMEG_0358, aftD, and MSMEG_0360 transcripts in WT M. smegmatis mc2155 and the aftD single crossover strain SCO1. One quarter of the RT-PCR reactions were analyzed on 1% agarose gels.