Table 2.
Types and frequency (x10-5) of lacZα mutations induced by HNE treatment of dsDNA of M13mp18 phage and allowing it to replicate in E. coli wild-type and mutants defective in NER and/or recA-dependent recombination.
| Type of mutation | Spontaneous mutations | HNE-induced mutations | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| JM105 (wild-type) | JM105 (wild-type) | BH1100 (recA) | BH430 (uvrA) | BJ1 (uvrArecA) | ||||||
| mutation frequency x10-5 | % | mutation frequency x10-5 | % | mutation frequency x10-5 | % | Mutation frequency x10-5 | % | mutation frequency x10-5 | % | |
| Base substitutions | 33.3 | 61.3 | 108 | 80 | 164.6 | 84.2 | 217.2 | 48 | 288 | 94.7 |
| Frameshifts | ||||||||||
| - insertions | nd* | 9.7 | 6.75 | 10 | nd | 5.3 | 18.8 | 4 | 16 | 5.3 |
| - deletions | 6 | 6.75 | 10.3 | nd | nd | |||||
| Recombination deletion of 93+54 nucleotides | 3.5 | 6.5 | 13.5 | 10 | 20.6 | 10.5 | 217.2 | 48 | nd | nd |
| Other deletions | 12 | 22.5 | nd | nd | nd | nd | nd | nd | nd | nd |
| Total | 54.8 | 100 | 135 | 100 | 195.5 | 100 | 453.2 | 100 | 304 | 100 |
*nd - not detected