Fig. 5.

Diurnal profiles in the amount of transcript (a–f) and of protein (g) of components of the ribbon complex in the rat pineal gland. a–f Amount of transcript of RIBEYE (a), Bassoon (b), Piccolo (c), Munc13–1 (d), KIF3A (e), arylalkylamine N-acetyltransferase (AA-NAT; f) as a function of light onset (solid bars dark period under the 12:12 light/dark regime, ZT Zeitgeber time). Significant 24-h oscillation is only found in the transcript amount of AA-NAT (analyzed by one-way ANOVA: P<0.001). Data represent a percentage of the maximal value of the light/dark cycle. Each value represents the mean±SEM (n=3; each derived from two or three pooled pineal glands). g Western blot analysis showing immunoreactivities of protein components of the ribbon complex. Each lane represents an individual rat pineal gland 7 hours after light onset (L, n=3) or after dark onset (D, n=3). Lanes were loaded with 15 μg of total protein. The proteins under investigation are equally expressed at day and night