Fig. 3.

Effect of DCMU on the induction of the P_HSP70A-LUC reporter construct by H₂O₂ in Chlamydomonas transformants. Cells were incubated in the presence of 6 μM DCMU for 45 min prior to the addition of 2 mM H₂O₂. After H₂O₂ addition, cells were incubated for 5 h in the light (70 μmol photons m⁻² s⁻¹) or in the dark. Luciferase activity was normalized with respect to cell numbers. The luciferase activity of light-grown cultures in the absence of DCMU was set as one