Fig. 8.

Effect of aminotriazole and DCMU on the H₂O₂ induction of the P_HSP70A-LUC reporter gene at the RNA level in Chlamydomonas transformants. Cultures incubated in the dark (D) were treated with H₂O₂ (2 mM final concentration) in the presence (lane 4) or absence (lane 3) of the catalase inhibitor aminotriazole (2 mM AT). AT was added 45 min prior to the addition of H₂O₂. Cells incubated in the light (L) were treated with H₂O₂ (2 mM final concentration) in the presence (lane 5) or absence (lane 2) of the PSII inhibitor DCMU (6 μM). DCMU was added 45 min prior to the addition of H₂O₂. A control culture that did not receive H₂O₂ was incubated in the light (lane 1). Samples for RNA isolation were taken 2 h after H₂O₂ addition. RNA blots (10 μg total RNA per lane) were hybridized with a probe specific for the LUC transgene and CBLP, the latter serving as a loading control as described in Sect. “Materials and methods”