Table 2.
Item | Price in EUR for 900 collected samples |
---|---|
Collection materials for 900 samples, following the two-step collection method (Nsubuga et al. 2004) | 620 |
DNA extraction with QIAamp DNA stool mini kit | 2,800 |
DNA quantification using quantitative PCR (Morin et al. 2001) | 1,200 |
Sexing using the amelogenin assay with three replicates for each sample | 500 |
Performing PCRs at 15 loci with three replicates for each sample | 7,600 |
Genotyping using the ABI sequencer with three dyes loaded at each time | 3,600 |
Total | 16,320 |
In this example, the population to be studied consists of an estimated 300 individuals unhabituated to human observation. To reliably estimate the population size or to confirm an individual’s identity from multiple samples, three samples are assumed to have been collected for each individual. Furthermore, each sample is assumed to be genotyped three times at each locus, including the sexing locus. This procedure is highly conservative but is good practice in studies utilizing noninvasive samples. The prices used for calculations are as of 2008 in Germany. Salaries for laboratory personnel are not included