Table 2.
Nomenclature and characteristics of rye Cbf genes cloned from the cultivar Puma rye
| Gene name | Genbank accession number | Group | Chromosome location | Sequence length (bp) | Protein length (aa) | pI | MW (kDa) | Best BLAST | Identity (%) |
|---|---|---|---|---|---|---|---|---|---|
| ScCbfIa-11 | EU194240 | Ia | 2R | 614 | 204 | 5.60 | 23.8 | TaCbfIa11 | 92.9 |
| ScCbfII-5 | EU194241 | II | – | 641 | 213 | 6.06 | 23.8 | TaCbfII-5.2 | 86.0 |
| ScCbfIIIa-6 | EU194242 | IIIa | 5RL | 689 | 229 | 4.92 | 25.9 | TaCbfIIIa-D6 | 91.2 |
| ScCbfIIIc-10 | EU194243 | IIIc | 5RL | 666 | 221 | 4.80 | 24.8 | TmCbf10 | 92.4 |
| ScCbfIIIc-3A | EU194244 | IIIc | 5RL | 686 | 228 | 4.57 | 25.7 | TaCbfIIIc-D3 | 94.6 |
| ScCbfIIIc-3B | EU194245 | IIIc | 5RL | 695 | 231 | 4.61 | 25.8 | TaCbfIIIc-D3 | 94.0 |
| ScCbfIIId-12 | EU194246 | IIId | 5RL | 698 | 232 | 5.70 | 26.2 | TaCbfIIId-B12 | 93.7 |
| ScCbfIIId-15 | EU194247 | IIId | 5RL | 662 | 220 | 5.11 | 24.8 | TaCbfIIId-A15 | 93.4 |
| ScCbfIIId-19 | EU194248 | IIId | 5RL | 665 | 221 | 5.13 | 25.2 | TaCbfIIId-D19 | 94.4 |
| ScCbfIVa-2Aa | EU194249 | IVa | 5RL | 605 | >193 | (5.76) | (22.0) | TaCbfIVa-A2 | 94.7 |
| ScCbfIVa-2Ba | EU194250 | IVa | 5RL | 651 | >193 | (5.98) | (22.0) | TaCbfIVa-A2 | 94.3 |
| ScCbfIVb-20b | AF370728 | IVc | 5RL | 850 | 212 | 8.53 | 23.3 | TaCbfIVb-A20 | 91.4 |
| ScCbfIVd-9Ab | AF370729 | IVd | 5RL | 1040 | 268 | 9.47 | 28.9 | TaCbfIVd-B9 | 90.9 |
| ScCbfIVd-9Bb | AF370730 | IVd | 5RL | 1145 | 270 | 9.00 | 29.2 | TaCbfIVd-B9 | 92.8 |
aCloned sequences were truncated at their 5′ ends (lacked the first 78 bp based on their wheat ortholog). Partial protein sequences were used to calculate pI and MW values (in brackets)
bPreviously reported by Jaglo et al. (2001)