Fig. 1.

APC suppressed apoptosis and induced activation of p38 and Akt kinases. a Number of apoptotic EC (Hoechst-33342 staining) and western blot analysis of cleaved caspase-3 under control conditions (no treatment) or after 2 h of simulated ischemia (SI) without or with APC treatment. Values are mean ± SEM, n = 5–6. *P < 0.05 versus SI. b Western blot analysis of phospho-ERK1/2, phospho-SAPK/JNK, phospho-p38 and phospho-Akt kinases performed with whole cell lysates prepared from control EC (Con) or from EC after 10 min of APC. APC led to a marked increase in phosphorylation of Akt and p38, but not ERK1/2 or SAPK/JNK kinases. c Western blot analysis of phospho-p38 and phospho-Akt at different time (10–40 min) during APC. d Western blot analysis of p38-alpha and phospho-p38-alpha in control EC (Con) or in EC after 10 min of APC. e Western blot analysis of p38-beta in control rat cardiomyocytes (CM) or in control rat coronary EC. Note that no p38-beta protein could be detected in EC. Western blot data are representative of three to five independent experiments with similar results