Table 2.
Oligonucleotides used in this study
| Primer | Sequence (5′→3′) | Site |
|---|---|---|
| Library construction | ||
| Eag_fwd | CTGAACTCGCTGAAGATAAC | |
| Eag_rev | GGCCGTTATCTTCAGCGAGTTCAG | |
| Gene disruption | ||
| REP1-lb_Fwd | TTTGCGTATTCCACCTGCAGTAGCC | |
| REP1-lb_Rev | CACGGCCTGAGTGGCCAAGAGAGTGTGATTCTTGCGAGCGG | SfiI |
| REP1-rb_Fwd | GTGGGCCATCTAGGCCTGCTTGCAGATCGCTATGCAGATGG | SfiI |
| REP1-rb_Rev | CAACTACTGGGAAAAGTATGGAGCGG | |
| HUM2-lb_Fwd | ACATTCAGCAAACAGCAAATGACCC | |
| HUM2-lb_Rev | CACGGCCTGAGTGGCCGCTGAAGAGCTAGAGAGTGTGGTTGG | SfiI |
| HUM2-rb_Fwd | GTGGGCCATCTAGGCCGTCGTGACTGCTCGCTCTCTTTCC | SfiI |
| HUM2-rb_Rev | TGACGTGCTGGCTAAGTTGTCGC | |
| RPH1-lb_Fwd | CGGAAAGGGATGTCTTGGTTGTTAC | |
| RPH1-lb_Rev | CACGGCCTGAGTGGCCAGGCAGTTGATTGGTGTTTGGATAG | SfiI |
| RPH1-rb_Fwd | GTGGGCCATCTAGGCCTTTGGTTCGCATTCTGGTTTCGTC | SfiI |
| RPH1-rb_Rev | ATGTGAAGTACAAACTTCGGCGTGC | |
| RSP1-lb_Fwd | ACCGAGGCTATGGTTCTTCTAGTCC | |
| RSP1-lb_Rev2 | CACGGCCTGAGTGGCCGTGAAGAGATGCTGCTGCGAGAGG | SfiI |
| RSP1-rb_Fwd2 | GTGGGCCATCTAGGCCTGTACGCTCTTTCTCGCTCACAACC | SfiI |
| RSP1-rb_Rev | TCTACTAACCGAAGGCTCTGACCTGG | |
| RSP2-lb_Fwd | TCTCCCACACTAACCTGAATGAGAGC | |
| RSP2-lb_Rev | CACGGCCTGAGTGGCCTATAAGAGGTTGGTGACGATGGTGG | SfiI |
| RSP2-rb_Fwd | GTGGGCCATCTAGGCCCTGGTTGTGCTTCGTTTTAGTTTGC | SfiI |
| RSP2-rb_Rev | AAGCAGAATCTGGTCCATACAAATCG | |
| eGFP fusion | ||
| RPH1-GFP_Fwd | GAGAGAGGATCCATGAAGTACCTTCAGTTCCTCGCTG | BamHI |
| RPH1-GFP_Rev | GAGAGACCATGGAGTTGATAGGGATCGAAGTGCAGC | NcoI |