Table 3.
Identification of secreted proteins by yeast signal sequence trap (YSST) screening combined with bioinformatic analysis
| YSST | Acc | Prediction | Annotation | ||
|---|---|---|---|---|---|
| NN | HM | PC | |||
| 1 | UM05222 | S | S | S | Hypothetical protein |
| 2 | UM10301 | S | S | S | Hypothetical protein |
| 11 | UM11562 | S | S | S | Hydrophobin (Hum2) |
| 13 | UM06162 | S | S | S | Immunoreactive mannoprotein (MP88) |
| 14 | UM02295 | S | S | S | Hypothetical protein |
| 17 | UM03138 | S | S | S | Hypothetical protein |
| 27 | UM05295 | S | S | S | Hypothetical protein |
| 29 | UM01513 | S | S | S | Riboflavin-aldehyde forming enzyme |
| 30 | UM03392 | S | S | S | Hypothetical protein |
| 31 | UM04035 | S | S | S | Hypothetical protein |
| 33 | UM05622 | S | S | S | Conserved hypothetical protein |
| 34 | UM01202 | O | S | S | Hypothetical protein |
| 36 | UM00876 | S | S | S | Exo-1,3-beta-glucanase |
| 46 | UM04433 | S | S | S | Hydrophobin (Hum3) |
| 52 | UM01014 | S | S | S | Thioredoxin related protein |
| 53 | UM05953 | S | S | S | Hypothetical protein |
| 56 | UM00310 | S | S | S | Related to WD-repeat protein crb3 |
| 62 | UM01165 | S | S | S | Related to Glucan 1,3-beta-glucosidase precursor |
| 63 | UM03065 | S | S | M | Putative protein |
| 67 | UM11266 | S | S | S | Probable lysophospholipase (lpl) |
| 73 | UM01238 | S | S | S | Hypothetical protein |
| 74 | UM06218 | S | S | O | Transglycosylase SLT domain protein |
| 77 | UM03923 | S | S | S | Hypothetical protein |
| 83 | CD488380a | S | S | S | Hypothetical protein |
| 101 | UM03924 | S | S | S | Repellent protein 1 precursor (Rep1) |
| 102 | UM03046 | S | S | S | Hypothetical protein |
| 142 | UM06112 | S | S | S | Hypothetical protein (Rsp1) |
| 162 | UM00904 | S | S | S | Related to glucose regulated stress protein, Hsp70-like |
| 169 | UM01976 | S | S | S | Hypothetical protein |
Accession numbers (Acc.) according to MUMDB, release 11–2005 (http://mips.gsf.de/genre/proj/ustilago/). Prediction: Sequences were analyzed with SignalP (v. 3.0) algorithms (neural network, NN, and hidden markov model, HM) and protcomp (v. 6.0; http://www.softberry.com, PC)
S secretory pathway, M mitochondrial protein, O other localization
aEST library of germinating teliospores (Sacadura and Saville 2003)