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. Author manuscript; available in PMC: 2010 Oct 5.
Published in final edited form as: Anal Chim Acta. 2009 Aug 27;651(2):201–208. doi: 10.1016/j.aca.2009.08.032

Figure 2. High resolution NMR spectra of a methanolic extract of LCC2 cells.

Figure 2

Figure 2

Figure 2

Glycerophospholipids were extracted from LCC2 cells grown in the presence of 10 mM [U-13C]- glucose for 24 h as described in the text. NMR spectra were recorded at 800 MHz, 20°C as described in the methods.

A,B. TOCSY spectrum recorded with a mixing time of 50 ms with 1D 1H NMR spectrum. The TOCSY spectrum was recorded at 18.8 T 293 K with 50 ms mixing time at a B1 field strength of 9 kHz. The data were processed with one linear prediction and zerofilling in t1 and apodized using an unshifted Gaussian function in both dimensions.

C. 1D NMR spectra

Top: 1D 13C-edited 1H (HSQC) spectrum

Bottom: high resolution 1H NMR spectrum