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. Author manuscript; available in PMC: 2009 Oct 6.
Published in final edited form as: Vaccine. 2007 Feb 23;25(19):3773–3780. doi: 10.1016/j.vaccine.2007.02.010

Fig. 2.

Fig. 2

Intranasal or i.p. CPAF+CpG vaccination induces robust humoral immune responses. Animals (6 mice/group) were treated i.n. or i.p. with CPAF + CpG, CPAF + ODN, CpG, or PBS on days 0, 14, and 28. Sera or vaginal fluids were collected from animals two weeks after final immunization. (A) Systemic anti-CPAF Ab responses after immunization. Serum anti-CPAF antibody levels were analyzed by ELISA using CPAF-coated microtiter plates. Results are expressed as mean ± SD of reciprocal serum dilutions corresponding to 50% maximal binding. (B) Mucosal anti-CPAF IgA responses after immunization. Results are expressed as mean ± SD of the absorbance of undiluted vaginal fluids. * Significant differences between CPAF+CpG and CPAF+ODN immunized animals (P < 0.05, Kruskall-Wallis test). Results are representative of two independent experiments.