Fig. 4.

CPAF+CpG vaccination reduces the development of oviduct pathology. Animals (6 mice/group) were treated i.n. or i.p. with three doses of CPAF + CpG, CPAF + ODN, or PBS. One month following final vaccination, mice were challenged i.vag. with 5 × 10⁴ IFU of C. muridarum. At day 80 following challenge, animals were euthanized and tissues collected for further analysis. (A) Percentage of animals developing hydrosalpinx in different immunization groups after genital chlamydial challenge: B-bilateral and U-unilateral. (B) Quantitative histopathological scoring of oviduct dilatation. (C) Quantitative estimation of cellular infiltration into the genital tracts following chlamydial challenge. Means ± SD of histopathology and cellular infiltration scores are shown. * Significant differences between CPAF+CpG and mock-immunized (PBS) animals (P < 0.05, Mann-Whitney Rank-Sum test). Results are representative of two independent experiments.