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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Cancer Prev Res (Phila). 2009 Jan 13;2(2):134–142. doi: 10.1158/1940-6207.CAPR-08-0062

Figure 5. Dose-responsive effects of red clover isoflavones to inhibit DHEA+TGFβ-1-induced PSA expression in LAPC-4 cell monocultures and cocultures and T metabolism in 6S stromal cell monocultures and cocultures.

Figure 5

A. The same experimental procedure for PSA ELISA was used as in Figures 2 and 4, but in addition to hormone treatments of 100 nM DHEA +/- 40 pM TGFβ1, 10 nM R1881, cells were also treated with DHEA + TGFβ1 + RC isoflavones at 10nM, 30nM, 100nM or 300nM. B. Testosterone concentrations were determined in conditioned media from stromal cell monocultures, compared to cocultures from the same experiments represented in Figure 5A. Hormone treatments included 100 nM DHEA +/- 40 pM TGFβ1, DHEA/TGFβ1 + RC isoflavones at 10nM, 30nM, 100nM or 300nM; and10 nM R1881. Data are expressed as the mean and SE from three experiments. * p = 0.05; ** p = 0.01;*** p = 0.001; + p=0.05; ++ p=0.01; +++ p=0.001 ◆ p = 0.05 compared with DHEA + TGFβ1 alone.