A. The same experimental procedure for PSA ELISA was used as in Figures 2 and 4, but in addition to hormone treatments of 100 nM DHEA +/- 40 pM TGFβ1, 10 nM R1881, cells were also treated with DHEA + TGFβ1 + RC isoflavones at 10nM, 30nM, 100nM or 300nM. B. Testosterone concentrations were determined in conditioned media from stromal cell monocultures, compared to cocultures from the same experiments represented in Figure 5A. Hormone treatments included 100 nM DHEA +/- 40 pM TGFβ1, DHEA/TGFβ1 + RC isoflavones at 10nM, 30nM, 100nM or 300nM; and10 nM R1881. Data are expressed as the mean and SE from three experiments. * p = 0.05; ** p = 0.01;*** p = 0.001; + p=0.05; ++ p=0.01; +++ p=0.001 ◆ p = 0.05 compared with DHEA + TGFβ1 alone.