Figure 6. Akt phosphorylation is elevated in dermal fibroblasts and is reduced by rac inhibition.

Whole cell lysates were subjected to SDS/PAGE and Western blot analysis with an anti-phospho Akt and anti-Akt antibodies in the absence (TOP PANEL) or presence or absence (BOTTOM PANEL) of 50 µM NSC23766. Quantitative densitometry data is indicated on the right. Experiments were performed on cells derived from 6 normal individuals and 6 SSc patients. Quantitative densitometry data is indicated on the right (* = p<0.05 relative to wild-type control).