Figure 5.

Nitration of mitochondrial proteins in response to acute organic nitrate treatment. Protein tyrosine nitration was tested in isolated cardiac mitochondria (0.2 mg protein mL⁻¹) from control mice for glyceryl trinitrate (GTN) (5–5000 µM) (A) and 2-nitrooxyethylammoniumnitrate (AEN), methyl-3-nitrooxypropanoat (NPME), triethanolamine trinitrate (TEAN) (5 mM) (B). 3-Nitrotyrosine formation was detected by dot blot analysis using a specific antibody. Dimethyl sulphoxide (DMSO) was used as a solvent control for AEN, NPME and TEAN whereas ethanol (EtOH) was used for GTN respectively; 20 µg of protein was transferred to each well. Below the densitometric quantification the original blot is shown. Data shown in (B) are mean ± SEM of eight independent experiments. *P < 0.05 versus DMSO solvent control.