Figure 8.

Mitochondrial reactive oxygen species (ROS) formation after chronic organic nitrate treatment. Isolated cardiac mitochondria (0.1 mg protein mL⁻¹) where stimulated with succinate (5 mM), the chemiluminescence signal was detected using a single photon counter in the presence of the dye L-012 (100 µM). The organic nitrates 2-nitrooxyethylammoniumnitrate (AEN), triethanolamine trinitrate (TEAN) and glyceryl trinitrate (GTN) were infused at a dose of 150, 140 or 50 µg·h⁻¹ for 3 days respectively. Data are mean ± SEM of 24 (AEN), 20–21 (TEAN) and 13–17 (GTN) independent experiments with mitochondria from at least eight animals per group. *P < 0.05 versus solvent (sham-treated) control.