Figure 6.

Effects of NCX 2057 and ferulic acid (FA) on cyclooxygenase type-1 (COX-1) and cyclooxygenase type-2 (COX-2) activities in RAW macrophages. Panels A and B show the inhibitory profile of NCX 2057 and FA on COX-1 and COX-2 respectively. Values represent the mean ± standard error of mean of three independent experiments each conducted in triplicate. Experiments were performed in presence of 1 µM of the enzyme substrate, arachidonic acid and the extent of drug-induced inhibition was estimated from the amount of prostaglandin E₂ (PGE₂) production. Panels C and D are representative Lineweaver–Burke plots for COX-1 and COX-2 enzymes in the absence, in the presence of NCX 2057 at 30 µM or FA at 30 µM.