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. 2009 Oct 13;4(10):e7443. doi: 10.1371/journal.pone.0007443

Figure 5. GSK3β inhibition reduced the CD133+ cell population of a GBM cell line.

Figure 5

(A) LN319 GBM cell line was treated for 72 h with 10 or 20 mM LiCl or with SB216763 at 10 or 20 µM. GSK3β inhibition reduced the CD133-positive (CD133+) population. (B) LN319 CD133+ cells were enriched to 80% purity by cell sorting. The enriched population was treated for 72 h with 10 or 20 mM LiCl or with SB216763 at 10 or 20 µM. (C) Western blot analysis of Notch2, Nestin, Bmi1, β-catenin, GSK3β and β-actin in CD133+ and CD133-negative (CD133-) enriched populations compared with the control. **P<0.01; ***P<0.001; one-way ANOVA (Newman-Keuls Multiple Comparison Test).